The section of Clinical Chemistry of the Department of Pathology offers a wide range of examinations utilizing automated (wet & dry chemistry analyzers) and semi automated methods. It concentrates mainly on the chemical analysis of various metabolites, electrolytes and enzymes not only in the serum but in other body fluids as well.
In support to the National Reference Laboratory for Clinical Chemistry, the section also offers Referral, Confirmatory, Surveillance and Research Services as mandated by Department Order #393-E s.2000.
As National Reference Laboratory for Clinical Chemistry, the section also participates in an international External Quality Assessment Scheme like the UK, NEQAS in Clinical Chemistry and BIORAD EQAS.
The Section of Hematology of the Department of Pathology and Laboratory is a section that can service all departments including the Hematology-Oncology as well as consultative services in the diagnosis of various hematologic diseases and disorders.
The section is linked-up with the Thoracic-Oncology program, minimally invasive Thoracic surgery for Pediatric patients as well as Hospice and Palliative care.
Specialized procedures and services for the diagnosis and management of blood diseases as well as routine and specific tests for White Blood Cells, Red Blood Cells, Platelets and other protein analysis are provided accordingly. Bleeding and clotting disorders requiring comprehensive evaluation and procedure is also provided by this unit.
Special Procedure for BLOOD BANK
- Platelet Pheresis
- Therapeutic Plasma Exchange
- Patient's needing blood transfusion are given safe
and reliable units
- Highest priority in the health and safety of our
Donor's, patients, executing safe
- Creating work environment that promote integrity,
caring for people and teamwork
Histopathology Section provides full range of timely and high quality histopathological and cytological services. These services include routine processing, sectioning and staining of tissues for microscopic examination, performing various special stains and immunohistochemistry for diagnostic and research specimens. The section also provides rapid diagnosis through Fresh Frozen Section and Rapid Pap's Cytology.
With highly trained pathologists and superior technical expertise of histopathology staff, the histopathology section is the National Referrence Laboratory for Anatomic Pathology specializing in pulmonary cases.
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The Section of Microbiology of the Department of Pathology and Laboratory offers examinations for the diagnosis of infections caused by bacteria, fungi, mycobacteria, as well as emerging and re – emerging diseases. Most of the specimens received in the section are from respiratory sources (sputum, ETA, pleural fluid, bronchial aspirates) with the addition of other body fluids (CSF, synovial fluid) and tissues.
Bacteriology performs culture, identification and drug sensitivity testing using manual and automated methods. The latest addition to the arsenal is the acquisition of the Vitek 2 System that provides automated identification of the organism as well as sensitivity to antibiotics. After the organism is grown in agar media, it is processed using the Vitek cartridge and in a matter of 8 hours the result of the identification and sensitivity testing is already available.
Diagnostic procedures for tuberculosis are done in the TB Laboratory which is housed in the National Center for Pulmonary Research. Direct sputum smear microscopy, TB culture and sensitivity testing are just some of the tests done in the TB laboratory. Recent trends in the diagnosis of diseases have shifted attention to molecular diagnostics and the diagnosis of TB is no exception. The TB laboratory offers molecular diagnostic procedures for TB which includes TB PCR (Polymerase Chain Reaction) genotyping and the Genexpert MTB – Rif Assay. The TB PCR (Figure1.) detects the presence of a gene found only in TB in specimens such as sputum, pleural fluid, body fluids, and tissue. Results are available within 5 working days (Figure 2.).
Figure 1. TB Polymerase Chain Reaction (PCR) kit and machine
Figure 2. Result of TB – PCR
The Genexpert MTB – Rif assay (Figure 3.) is another PCR – based test that also detects the presence of TB in specimens with the additional detection of resistance to Rifampicin. Another additional feature of the test is its simplicity and speed, with results being made available within 24 hours (Figure 4).
Figure 3. Genexpert Dx Machine and Cartridge
Figure 4. Result printout for Genexpert MTB – Rif Assay
More recently, diagnosis of emerging and re – emerging disease such as AH1N1 and MERS - CoV has been added to the services of the Section of Microbiology since the institution was chosen by the Department of Health (DOH) to be one of the 5 Sub - National laboratories (SNLs). Personnel are trained to use PCR methods to detect the presence of the above – mentioned diseases.
SPECIAL PATHOGEN LABORATORY (SPL)
Pursuant to Department Personnel Order No. 2009-2441. The Lung Center of the Philippines (LCP) Department of Pathology-Laboratory in 2009 was tapped to assit the Research Institute for Tropical Medicine (RITM) in the accurate detection of Pandemic Influenza AH1N1 using the technology of Real time RT-PCR.
In recent years, novel respiratory pathogens have emerged, including Avian Influenza (AH5N1 in 2005, AH7N9 in 2013) and Middle East Respiratory Syndrome – Coronovirus (MERS – CoV) in 2012.
Likewise, relative to Department Memorandum No. 2014-0365 – "Designation of the Lung Center of the Philippines as the Referral Center for ZEmerging and Re- Emerging Respiratory Tract Diseases like the Middle East Respiratory Syndrome – Coronavirus (MERsCoV)" and in order to carry out the mandated function as such, the Department of Pathology has established the Special Pathogen Laboratory to cater to the needed laboratory services for the detection of emerging and re-emerging diseases. The Special Pathogen Laboratory also plays a part in the Surveillance of Severe Acute Respiratory Infections (SARI) and Influenza Like Illnesses (ILI) in the Philippines as one of its Sentinel sites.
With proficient staff and state of the art equipment, the Special Pathogen laboratory is capable of detecting the above – mentioned novel, emerging and re-emerging pathogens by Real time PCR.
The Section offers a promising area of hope for the amelioration of many diseases for which there has previously been no cure and minimal hope of prevention.
The Stem Cell Laboratory provides quality, autologous dendritic cell vaccines and other autologous adult stem cell-based therapies. For active immunotherapy, dendritic cells are used to trigger the immune response against diseases including cancer. For regenerative purposes, autologous adult stem cells are administered to mediate repair of affected tissues.
The following are the services offered by the Molecular Diagnostics and Cellular Therapeutics Laboratory (Stem Cell unit):
- Immune Cell Therapy (Dendritic Cell Therapy)
- Circulating Tumor Cell Count
- Regenerative Therapy (MSC, EPC)
- 3 and 6 Gene Expression (Tumor Gene Expression Panel)
- EGFR Test (Roche)
- EGFR Test (Astra Zenica
The EGFR test is used primarily to help guide treatment and determine whether a patient with non-small cell lung cancer (NSCLC) may benefit from targeted therapy. This testing detects the presence activating mutations in the EGFR gene in the DNA of cells in tumor tissue.
The section of clinical microscopy being the most basic component of the Clinical Laboratory supports diagnosis of various diseases and medical conditions. The tests involves with the physiochemical microscopic analysis of various body fluids. These examinations are performed manually and/automated utilizing various reagents and kits. Among the most common tests include:
- Pregnancy tests (Urine/Serum)
- Occult blood test in stool
- Sperm Analysis
- Body Fluid analyses (pleural, synovial, ascitic, cerebrospinal)
- Concentration Techniques for parasitic infestation
IMMUNOLOGY AND SEROLOGY
The section of immunology and serology is focused on the services supporting the diagnosis of Infectious Diseases utilizing both automated and manual serologic methods. The section is not only involved in diagnostic serology but also do collaborative work with the Blood Bank section in the screening of blood units for Infectious and Blood borne diseases. Currently, the following are the tests being done by the section:
- Anti-HIV screening test
- Particle Agglutination test for Syphilis
- Typhi-Rapid for Salmonella Typhi
- Rapid Dengue Test for Dengue Fever (NS1 Ag and IgM/IgG)
- Thyroid Markers: FT3, FT4, TSH
Out Patient Laboratory Services, a front line service of the Department of Pathology, provides a range of laboratory examination essential in the diagnosis and assessment of the health of patients. The services encompass arrangement for requisition, patient preparation, patient identification, collection of sample, send-out preparation to referral laboratory, research collaboration and timely releasing of laboratory results.
The aim of the Out Patient Laboratory Services is not only to provide accurate and fast results but to do with a respect for ethics, confidentiality and safety of the patient.
In-Patient: 24 hours Monday-Sunday
Out-Patient: 7:00am - 5:00pm Monday-Saturday
Pathology and Laboratory Department, Lung Center of the Philippines
Quezon Avenue, Quezon City, 1104 Philippines
Telephone Number: 924-6101